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8.000.6018EN Quantification of carbohydrates and uronic acids in EUCALYPTUS wood hydrolysates by ion chromatography with PAD using a gold electrode  
Quantification of carbohydrates and uronic acids in EUCALYPTUS wood hydrolysates by ion chromatography with PAD using a gold electrode

The purpose of this study was to identify and quantify various hemicellulosic sugars (arabinose, galactose, glucose, xylose and mannose) and uronic acids (glucuronic and galacturonic acids) in acidic hydrolysates of Eucalyptus dunnii and Eucalyptus SP woods. Separation of the monomeric sugars and the uronic acids is achieved on the high-capacity Metrosep Carb 1 – 250 ion-exchange column using a sodium hydroxide/sodium acetate eluent. Detection of the underivatized electro-active components occurs by pulsed amperometric detection (PAD) using a gold electrode. Because of the eluent’s low sodium hydroxide concentration, sugar analysis in the Eucalyptus wood hydrolysates includes post-column addition of sodium hydroxide (300 mmol/L) prior to detection.
Calibration curves obtained with sugar standards are linear over the range of 0.5…90 mg/L providing correlation coefficients better than 0.998. Relative standard deviations (RSD) for retention time and the used peak signal are smaller than 0.07 and 4.3% respectively.
The experiments show that the main component of analyzed Eucalyptus dunnii wood hydrolysate is glucose (32.67%) followed by xylose (9.89%) and galactose (1.04%). The content of arabinose and mannose is smaller than 1%. In all three hydrolyzed Eucalyptus SP samples the major constituent is glucose (60…71%) followed by xylose (11…25%), arabinose (1...2%) and galactose (0.6…1.4%). Concentrations of galacturonic and glucoronic acid in Eucalyptus SP samples are below 2%.


English:  tp-ic-carbohydrates.pdf (828 KB)

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Ion Chromatography > Ion Exchange Chromatography > Anions - Electrochemical detection
   Quantification of carbohydrates and uronic acids in <EM>EUCALYPTUS</EM> wood hydrolysates by ion chromatography with PAD using a gold electrode